Fetal bovine bone cells synthesize bone-specific matrix proteins.

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Fetal bovine bone cells synthesize bone-specific matrix proteins

We isolated cells from both calvaria and the outer cortices of long bones from 3- to 5-mo bovine fetuses. The cells were identified as functional osteoblasts by indirect immunofluorescence using antibodies against three bone-specific, noncollagenous matrix proteins (osteonectin, the bone proteoglycan, and the bone sialoprotein) and against type 1 collagen. In separate experiments, confluent cul...

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Evaluation of tissue-engineered bone constructs using rabbit fetal osteoblasts on acellular bovine cancellous bone matrix

AIM The aim of this study was to generate composite bone graft and investigate the rabbit fetal osteoblasts adhesion, proliferation and penetration on acellular matrices of cancellous bone. MATERIALS AND METHODS Acellular cancellous bone was prepared and developed as in the previous study with little modification. These matrices were decellularized by rapid freeze and thaw cycle. To remove th...

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Enhancing Ectopic Bone Formation in Canine Masseter Muscle by Loading Mesenchymal Stem Cells onto Natural Bovine Bone Minerals.

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Rabbit bone marrow response to bovine osteoinductive proteins and anorganic bovine bone.

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ژورنال

عنوان ژورنال: Journal of Cell Biology

سال: 1984

ISSN: 0021-9525,1540-8140

DOI: 10.1083/jcb.99.2.607